Basic Usage & Preprocessing
29 October 2024
Preparation
## As sesame and sesameData are under active development, this documentation is
## specific to the following version of R, sesame, sesameData and ExperimentHub:
sesame_checkVersion()## SeSAMe requires matched versions of R, sesame, sesameData and ExperimentHub.
## Here is the current versions installed:
## R: 4.5.0
## Bioconductor: 3.21
## sesame: 1.25.0
## sesameData: 1.23.0
## ExperimentHub: 2.15.0We recommend updating your R, ExperimentHub, sesameData and sesame to use this documentation consistently. If you have installed directly from github, please make sure the compatible ExperimentHub is installed.
If you use a previous version, please checkout the vignette that corresponds to the right version here https://zhou-lab.github.io/sesame/dev/supplemental.html#Versions
CRITICAL: After a new installation, one must cache the associated annotation data using the following command. This needs to be done only once per SeSAMe installation/update. Caching data to local guarantees proper data retrieval and saves internet traffic.
This function caches the needed SeSAMe annotations. SeSAMe annotation data is managed by the sesameData package which uses the ExperimentHub infrastructure. You can find the location of the cached annotation data on your local computer using:
## [1] "/home/biocbuild/.cache/R/ExperimentHub"The openSesame Pipeline
The openSesame function provides end-to-end processing
that converts IDATs to DNA methylation level (aka β value) matrices in R. The function
input path_to_idats can be one of the following input:
- A path to a directory where the IDAT files will be recursively search
- Specific path(s) of IDAT file prefix, one for each IDAT pair
- One or a list of SigDF objects
The following code uses a directory that contains built-in two HM27
IDAT pairs to demonstrates the use of openSesame:
The BPPARAM= option is from the
BiocParallel package and controls parallel processing (in
this case, we are using two cores). Under the hood, the function
performs a series of tasks including: searching IDAT files from the
directory (the searchIDATprefixes function), reading IDAT
data in as SigDF objects (the readIDATpair
function), preprocessing the signals (the prepSesame
function), and finally converting them to DNA methylation levels (β values, the getBetas
function). Alternatively, one can run the following command to get the
same results, while gaining more refined control:
## The above openSesame call is equivalent to:
betas = do.call(cbind, BiocParallel::bplapply(
searchIDATprefixes(idat_dir), function(pfx) {
getBetas(prepSesame(readIDATpair(pfx), "QCDPB"))
}, BPPARAM = BiocParallel::MulticoreParam(2)))
## or even more explicitly (if one needs to control argument passed
## to a specific preprocessing function)
betas = do.call(cbind, BiocParallel::bplapply(
searchIDATprefixes(idat_dir), function(pfx) {
getBetas(noob(pOOBAH(dyeBiasNL(inferInfiniumIChannel(qualityMask(
readIDATpair(pfx)))))))
}, BPPARAM = BiocParallel::MulticoreParam(2)))The openSesame function is highly customizable. The
prep= argument is the same argument one gives to the
prepSesame function (see Data
Preprocessing for detail) which openSesame calls
internally. The argument uniquely specifies a preprocessing procedure.
The func= option specifies the signal extraction function.
It can be either be getBetas (DNA methylation) or
getAFs (allele frequencies of SNP probes) or NULL (returns
SigDF). The manifest= option allows one to
provide an array manifest when handling data from platform not supported
natively. Finally, the BPPARAM= argument is the same
argument taken by BiocParallel::bplapply to allow parallel
processing. See Supplemental
Vignette for details of these component functions of openSesame.
The output of openSesame can also be customized. It can
either be beta values, which are the end DNA methylation readings, as
shown above. It can also be a list of SigDFs which stores
the signal intensities and can be further put back to openSesame for
more processing. The openSesame(func=) argument specifies
whether the output is a SigDF list or beta values. The following shows
some usage:
betas = openSesame(idat_dir, func = getBetas) # getBetas is the default
sdfs = openSesame(idat_dir, func = NULL) # return SigDF list
allele_freqs = openSesame(idat_dir, func = getAFs) # SNP allele frequencies
sdfs = openSesame(sdfs, prep = "Q", func = NULL) # take and return SigDFsOne can also generate the detection p-values (e.g., for GEO upload)
by feeding openSesame with func = pOOBAH (or other
detection p-value calculators).
Data Preprocessing
The prep= argument instructs the openSesame
function to call the prepSesame function to preprocess
signal intensity under the hood. This can be skipped by using
prep="". The prepSesame function takes a
single SigDF as input and returns a processed
SigDF. When prep= is non-empty, it selects the
preprocessing functions (see Preprocessing Function
Code) and specifies the order of their execution. For example,
performs dye bias correction (D) followed by background
subtraction (B). In other words,
prepSesame(sdf, "DB") is equivalent to
noob(dyeBiasNL(sdf)). All the preprocessing functions take
a SigDF as input and return an updated SigDF.
Therefore, these functions can be chained together. The choice of
preprocessing functions and the order of their chaining is important
(see Supplemental
Vignette) for detailed discussions of these functions). The
following table lists the best preprocessing strategy based on our
experience.
| Platform | Sample Organism | Prep Code |
|---|---|---|
| EPICv2/EPIC/HM450 | human | QCDPB |
| EPICv2/EPIC/HM450 | non-human organism | SQCDPB |
| MM285 | mouse | TQCDPB |
| MM285 | non-mouse organism | SQCDPB |
| Mammal40 | human | HCDPB |
| Mammal40 | non-human organism | SHCDPB |
The optimal strategy of preprocessing depends on:
The array platform. For example, certain array platforms (e.g., the Mammal40) do not have enough Infinium-I probes for background estimation and dye bias correction, therefore background subtraction (where the out-of-band signals are from) might not work most optimally;
The expected sample property. For example, some samples have the signature bimodal distribution of methylation of most mammalian cells. Others may undergo global loss of methylation (germ cells, tumors etc). Other important factors include high-input vs low-input, tumor vs normal, somatic vs germ cells, human vs model organisms, mouse strains etc. Some platforms (e.g., Mammal40 and MM285) are designed for multiple species and strains. Therefore
SandTwould be important when those arrays are used on non-reference organisms (see Working with Nonhuman Arrays).
Preprocessing Function Code
The prepSesameList function lists all the available
codes and the associated preprocessing functions.
Here are some consideration when determining the preprocessing order.
Species (S) and strain (T) inference resets
the mask and color channels based on probe alignment and presence of
genetic variants. Therefore when they are used, they need to be called
first. Q masks non-uniquely mapped probes which may inflate
the out-of-band signal for background estimation. Therefore
Q should be used before detection p-value calculation
(P) and background subtraction (B) when
necessary. Channel inference (C) and dye bias correction
(D) should take place early since dye bias effect is
global. C should be placed before D because
dye bias correction uses in-band signal the identification of which
relies on correct channel designation. Detection p-value
(P) should happen before background subtraction
(B) since background subtraction modifies signal and may
affect out-of-band signal assumption used in P. Lastly,
functions that explicitly normalizes β value distribution
(M) should happen last if they even need to be used.
See Supplemental Vignette for details of preprocessing functions.
Lift over across platforms
To allow data integration across platforms, one can harmonize beta
values, probe ID list and even signal intensities, to a different
platform using the mLiftOver (methylation Lift Over)
utility. The following examples illustrates these corresponding
usages.
Project probe IDs
cg_msa = names(sesameData_getManifestGRanges("MSA"))
## only mappable probes, return mapping from MSA to HM450
head(mLiftOver(cg_msa, "HM450"))## cg13869341_BC11 cg12045430_BC11 cg18231760_BC21 cg17866181_BC21 cg08477687_BC21
## "cg13869341" "cg12045430" "cg18231760" "cg17866181" "cg08477687"
## cg23917638_TC21
## "cg23917638"cg_hm450 = names(sesameData_getManifestGRanges("HM450"))
cg_hm450 = grep("cg", cg_hm450, value=TRUE)
## only mappable probes, return mapping from HM450 to EPICv2
head(mLiftOver(cg_hm450, "EPICv2"))## cg00381604 cg21870274 cg06402284 cg23803172
## "cg00381604_BC11" "cg21870274_BC21" "cg06402284_TC21" "cg23803172_BC11"
## cg20788133 cg08258224
## "cg20788133_BC21" "cg08258224_TC21"Project beta values
betas = openSesame(sesameDataGet("EPICv2.8.SigDF")[[1]])
betas_epic = mLiftOver(betas, "EPIC", impute=FALSE)
length(betas_epic) # EPIC platform dimension## [1] 866553## [1] 152102## [1] 866553## [1] 0## use empirical evidence in mLiftOver
mapping = sesameDataGet("liftOver.EPICv2ToEPIC")
betas_matrix = openSesame(sesameDataGet("EPICv2.8.SigDF")[1:2])
dim(mLiftOver(betas_matrix, "EPIC", mapping = mapping))## [1] 539513 2## [1] 866553 2Collapse to cg prefixes
The more recent Infinium arrays (the mouse, EPICv2, and MSA arrays)
have suffixes to uniquely identify the probe design. If one prefers to
collapse methylation readings to the cg prefix so they are comparable to
the previous array generations, this can be done with
getBetas(..., collapseToPfx = TRUE). The same argument can
be passed to
openSesame(..., func=getBetas, collapseToPfx=TRUE)
betas = getBetas(sdf_from_EPICv2, collapseToPfx = TRUE)
## or
betas = openSesame("path_to_idats", collapseToPfx = TRUE)
## by default the method for collapsing is to make means
betas = openSesame("path_to_idats", collapseToPfx = TRUE, collapseMethod = "mean")
## one can also switch to min detection p-value
betas = openSesame("path_to_idats", collapseToPfx = TRUE, collapseMethod = "minPval")One may also use the betasCollapseToPfx function to
generate the traditional cg number readings.
Please find more info at the Supplemental documentation at the following [link] (https://zhou-lab.github.io/sesame/dev/supplemental.html#Replicate_Probes)
Session Info
## R Under development (unstable) (2024-10-21 r87258)
## Platform: x86_64-pc-linux-gnu
## Running under: Ubuntu 24.04.1 LTS
##
## Matrix products: default
## BLAS: /home/biocbuild/bbs-3.21-bioc/R/lib/libRblas.so
## LAPACK: /usr/lib/x86_64-linux-gnu/lapack/liblapack.so.3.12.0
##
## locale:
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## [5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
## [7] LC_PAPER=en_US.UTF-8 LC_NAME=C
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## time zone: America/New_York
## tzcode source: system (glibc)
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## attached base packages:
## [1] stats4 stats graphics grDevices utils datasets methods
## [8] base
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## [21] BiocGenerics_0.53.0
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